MICHIGAN STATE UNIVERSITY

NIH Microbiology Research Unit

Publications

2005

Fitch, B. R., K. L. Sachen, S. R. Wilder, M. A. Burg, D. W. Lacher, W. T. Khalife, T. S. Whittam, and V. B. Young. 2005. Genetic diversity of Campylobacter sp. isolates from retail chicken products and humans with gastroenteritis in Central Michigan. J Clin Microbiol 43:4221-4224.

Hyma, K. E., D. W. Lacher, A. M. Nelson, A. C. Bumbaugh, J. M. Janda, N. A. Strockbine, V. B. Young, and T. S. Whittam. 2005. Evolutionary genetics of a new pathogenic Escherichia species: Escherichia albertii and related Shigella boydii strains. J Bacteriol 187:619-628.

Wick, L. M., W. Qi, D. W. Lacher, and T. S. Whittam. 2005. Evolution of genomic content in the stepwise emergence of Escherichia coli O157:H7. J Bacteriol 187:1783-1791.

2006

Elena, S. F., T. S. Whittam, C. L. Winkworth, M. A. Riley, and R. E. Lenski. 2005. Genomic divergence of Escherichia coli strains: Evidence for horizontal transfer and variation in mutation rates. International Microbiol 8: 271-278.

Lacher, D. W., H. Steinsland, and T. S. Whittam. 2006. Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP. FEMS Microbiol Lett 261:80-87.

Mokhtar Arshad, M., H. A. Asmar, M. H. Rahbar, M. L. Bouton, E. Wells, M. J. Wilkins, and A. M. Saeed.  2006. Risk factors for Salmonella Oranienburg outbreak in a nursing home in Michigan. J Amer Geriatrics Soc 54: 715-717.

Monday, S. R., C. Keys, P. Hanson, Y. Shen, T. S. Whittam, and P. Feng. 2006. Produce isolates of the Escherichia coli Ont:H52 serotype that carry both Shiga toxin 1 and stable toxin genes. Appl Environ Microbiol 72:3062-3065.

Palaniappan, R. U., Y. Zhang, D. Chiu, A. Torres, C. Debroy, T. S. Whittam, and Y. F. Chang. 2006. Differentiation of Escherichia coli pathotypes by oligonucleotide spotted array. J Clin Microbiol 44:1495-1501.

Saeed, A. M., S. T. Walk, M. Arshad, and T. S. Whittam. 2006. Clonal structure and variation in virulence of Salmonella enteritidis isolated from mice, chickens, and humans. J AOAC Int 89:504-511.

Younus, M., M. J. Wilkins, M. M. Arshad, M. H. Rahbar, and A. M. Saeed. 2006. Demographic risk factors and incidence of Salmonella enteritidis infection in Michigan. Foodborne Pathog Dis 3:266-273.

Zhang, W., W. Qi, T. J. Albert, A. S. Motiwala, D. Alland, E. K. Hyytia-Trees, E. M. Ribot, P. I. Fields, T. S. Whittam, and B. Swaminathan. 2006. Probing genomic diversity and evolution of Escherichia coli O157 by single nucleotide polymorphisms. Genome Res 16:757-767.

2007

Arshad, M. M., M. J. Wilkins, F. P. Downes, M. H. Rahbar, R. J. Erskine, M. L. Boulton, and A. M. Saeed. 2007. A registry-based study on the association between human salmonellosis and routinely collected parameters in Michigan, 1995-2001. Foodborne Pathog Dis 4:16-25.

Arshad, M. M., M. J. Wilkins, F. P. Downes, M. H. Rahbar, R. J. Erskine, M. L. Boulton, M. Younus, and A. M. Saeed. 2007. Epidemiologic attributes of invasive non-typhoidal Salmonella infections in Michigan, 1995-2001. Int J Infect Dis. 2:89-94.

Bergholz, T. M., C. L. Tarr, L. M. Christensen, D. J. Betting, and T. S. Whittam.  2007.  Recent gene conversions between duplicated glutamate decarboxylase genes (gadA and gadB) in pathogenic Escherichia coli.  Mol Biol Evol 24:2323-2333.

Besser T. E., N. Shaikh, N. J. Holt, P. I. Tarr, M. E. Konkel, P. Malik-Kale, C. W. Walsh, T. S. Whittam, and J. L. Bono. 2007. Greater diversity of Shiga toxin-encoding bacteriophage insertion sites among Escherichia coli O157:H7 isolates from cattle than in those from humans.Appl Environ Microbiol 73:671-679.

Cho, S., D. J. Boxrud, J. M. Bartkus, T. S. Whittam, and M. Saeed. 2007. Multiple-locus variable-number tandem repeat analysis of Salmonella Enteritidis isolates from human and non-human sources using a single multiplex PCR. FEMS Microbiol Lett 275:16-23.

Feng, P. C., S. R. Monday, D. W. Lacher, L. Allison, A. Siitonen, C. Keys, M. Eklund, H. Nagano, H. Karch, J. Keen, and T. S. Whittam. 2007. Genetic diversity among clonal lineages within Escherichia coli O157:H7 stepwise evolutionary model. Emerg Infect Dis 13:1701-1706.

Lacher, D. W., H. Steinsland, T. E. Blank, M. S. Donnenberg, and T. S. Whittam. 2007. Molecular evolution of typical enteropathogenic Escherichia coli: clonal analysis by multilocus sequence typing and virulence gene allelic profiling. J Bacteriol 189:342-350.

Manning, S. D., R. T. Madera, W. Schneider, S. E. Dietrich, W. Khalife, W. Brown, T. S. Whittam, P. Somsel, and J. T. Rudrik. 2007. Surveillance for Shiga toxin-producing Escherichia coli, Michigan, 2001-2005. Emerg Infect Dis 13:318-321.

Mansfield, L. S., J. A. Bell, D. L. Wilson, A. J. Murphy, H. M. Elsheikha, V. A. Rathinam, B. R. Fierro, J. E. Linz, and V. B. Young. 2007. C57BL/6 and congenic interleukin-10-deficient mice can serve as models of Campylobacter jejuni colonization and enteritis. Infect Immun 75:1099-1115.

Walk, S. T., E. W. Alm, L. M. Calhoun, J. M. Mladonicky, and T. S. Whittam. 2007. Genetic diversity and population structure of Escherichia coli isolated from freshwater beaches. Environ Microbiol 9:2274-2288.

Walk, S. T., J. M. Mladonicky, J. A. Middleton, A. J. Heidt, J. R. Cunningham, P. Bartlett, K. Sato, and T. S. Whittam. 2007. The influence of antibiotic selection on the population genetic composition of Escherichia coli from conventional and organic dairy farms. Appl Environ Microbiol 73:5982-5989.

Younus M., E. Hartwick, A. A. Siddiqi, M. Wilkins, H. D. Davies, M. Rahbar, J. Funk, and M. Saeed. 2007. The role of neighborhood level socioeconomic characteristics in Salmonella infections in Michigan (1997-2007): assessment using geographic information system. Int J Health Geogr 6:56

2008

Tarr, C. L., A. M. Nelson, L. Beutin, K. E. Olsen, and T. S. Whittam. 2008. Molecular characterization reveals similar virulence gene content in unrelated clonal groups of Escherichia coli of serogroup O174 (OX3). J Bacteriol 190:1344-1349.

Ziebell, K., P. Konczy, I. Yong, S. Frost, M. Mascarenhas, A. M. Kropinski, T. S. Whittam, S. C. Read, and M. A. Karmali. 2008. Applicability of phylogenetic methods for characterizing the public health significance of Verocytotoxin-producing Escherichia coli (VTEC). Applied and Environmental Microbiology 74:1671-1675.

Manning, S. D, A. S. Motiwala, A. C. Springman, W. Qi, D. W. Lacher, L. M. Ouellette, J. M. Mladonicky, P. Somsel, J. T. Rudrik, S. E. Dietrich, W. Zhang, B. Swaminathan, D. Alland, and T. S. Whittam. 2008. Variation in virulence among clades of Escherichia coli O157:H7 associated with disease outbreaks. Proceedings of the National Academy of Sciences USA

Riordan JT, Viswanath SB, Manning SD, Whittam TS. Genetic differentiation of Escherichia coli O157:H7 clades associated with human disease by real-time PCR.
Journal of Clinical Microbiology, June 2008, p. 2070-2073, Vol. 46, No. 6

Eaton KA, Friedman DI, Francis GJ, Tyler JS, Young VB, Haeger J, Abu-Ali G, Whittam TS. The pathogenesis of renal disease due to enterohemorrhagic Escherichia coli in germ free mice. Infect Immun. 2008 Jul;76(7):3054-63

Schlager TA, Johnson JR, Ouellette LM, Whittam TS. Escherichia coli colonizing the neurogenic bladder are similar to widespread clones causing disease in patients with normal bladder function. Spinal Cord. 2008 Jun 17. [Epub ahead of print]

Konczy P, Ziebell K, Mascarenhas M, Choi A, Michaud C, Kropinski AM, Whittam TS, Wickham M, Finlay B, Karmali MA. Genomic O Island 122, Locus for Enterocyte Effacement and Evolution of Virulent Verocytotoxin-Producing Escherichia coli. J Bacteriol. 2008 Jun 27. [Epub ahead of print]