MI002-03 Research Center to Facilitate Study of STEC
This project is a request for continued support of the STEC Center to facilitate research on Shiga-toxin producing E. coli.In the past period of support, we established a repository for STEC cultures; developed a database of ~1700 STEC strains, representing a diversity of serotypes, geographic, and host sources; designed and implemented a website which includes a searchable database on virulence characteristics and molecular subtypes of STEC; assembled and distributed reference strain sets that comprise well-characterized isolates from patients with diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome as well as some strains from sick and healthy animals; developed and implemented both a multilocus sequence typing (MLST) and multilocus virulence gene profiling (MVGP) schemes for rapid identification and classification of pathogenic E. coli and Shigella.Here we propose two specific aims: 1) to evaluate the molecular subtyping schemes in well-designed case/control study of etiological agents of diarrhea disease. This work will be done in collaboration with Dr. Phillip Tarr, Washington University, St Louis, who will supply material from an ongoing case/control study of a pediatric population in Seattle.2) to characterize new STEC serotypes, outbreaks strains, and novel E. coli pathovars by MLST and MVGP. The long-term goal is to have a reliable and rapid DNA sequence-based classification schemes and databases for pathogens associated with food and waterborne diseases.
Publications:
Allelic subtyping of the intimin locus (eae )of pathogenic
Escherichia coli by fluorescent RFLP
Allelic Typing of the eae Locus of Pathogenic Escherichia coli by Fluorescent RFLP
Applicability of Phylogenetic Methods for Characterizing the
Public Health Significance of Verocytotoxin-Producing
Escherichia coli Strains
Characterization of the Escherichia coli O157:H7 Sakai GadE Regulon
Convergent Evolutionary Analysis Identifies Significant Mutations in
Drug Resistance Targets of Mycobacterium tuberculosis
Differentiation of Escherichia coli Pathotypes by Oligonucleotide
Spotted Array
Effect of Zinc in Enteropathogenic Escherichia coli Infection
Evolution of Genomic Content in the Stepwise Emergence of
Escherichia coli O157:H7
Evolutionary Genetics of a New Pathogenic Escherichia Species:
Escherichia albertii and Related Shigella boydii Strains
Gene Content and Evolution of Pathogenic Escherichia coli of the EHEC Clonal Complexes
Gene Expression Induced in Escherichia coli O157:H7 upon Exposure
to Model Apple Juice
Genetic Differentiation of Escherichia coli O157:H7 Clades Associated
with Human Disease by Real-Time PCR
Genetic Diversity among Clonal
Lineages within Escherichia coli
O157:H7 Stepwise
Evolutionary Model
Genomic diversity of pathogenic Escherichia coli of the EHEC 2
clonal complex
Genomic O Island 122, Locus for Enterocyte Effacement, and the
Evolution of Virulent Verocytotoxin-Producing Escherichia coli
Greater Diversity of Shiga Toxin-Encoding Bacteriophage Insertion
Sites among Escherichia coli O157:H7 Isolates from Cattle than
in Those from Humans
LEE-encoded regulator (Ler) mutants elicit serotype-specific protection,
but not cross protection, against attaching and effacing E. coli strains
Molecular Characterization Reveals Similar Virulence Gene Content
in Unrelated Clonal Groups of Escherichia coli of Serogroup
O174 (OX3)
Molecular Evolution of Typical Enteropathogenic Escherichia coli:
Clonal Analysis by Multilocus Sequence Typing and Virulence
Gene Allelic Profiling
Multilocus sequence typing (MLST) of Shiga toxin producing Escherichia coli associated
with disease in Michigan
Phylogenetic analysis of Clostridium botulinum
type A by multi-locus sequence typing
Population Structure of Non-0157 Shiga Toxin-Producing Escherichia coli (STEC) Associated With Disease in Michigan and Connecticut
Probing genomic diversity and evolution of Escherichia coli O157 by single-nucleotide polymorphisms
Produce Isolates of the Escherichia coli Ont:H52 Serotype That Carry
both Shiga Toxin 1 and Stable Toxin Genes
Recent Gene Conversions between Duplicated Glutamate Decarboxylase Genes
(gadA and gadB) in Pathogenic Escherichia coli
Virulence Profiling of Escherichia coli Isolates from Pediatric Hospital Patients
