MI001-03 Profiling the Emergence of Salmonella enteritidis - Completed
The overall goal of this project is to elucidate genetic and phenotypic changes underlying the shift in host species (mouse to chicken) and transmission path(trans-ovarian) in the emergence of Salmonella enterica serovar Enteritidis (SE). As part of this project, we will test the hypothesis that antibiotic resistance genes have epistatic effects on virulence and transmission, which can promote emergence and spread of epidemic clones. The specific aims are: 1) To assemble a collection of SE isolates from a variety of sources (including humans, chickens, eggs, and the environment) that spans the 3-decade period of dramatic increase in prevalence. Strains will be characterized by antimicrobial resistance patterns and virulence factors using in vitro assays and will be sorted into homogenous groups. 2) To assess the contribution of clonal turnover in SE emergence. Strains from selected groups will be characterized genetically using the sequences of conserved housekeeping genes, and DNA polymorphisms detected by genomic survey methods (PFGE, AFLP, and SNPs). 3) To establish a transovarian transmission model using chickens to measure the potential of SE subtypes to contaminate laid eggs.The transovarian model will be used in future projects for genetic analysis of the transmission process and the link to antibiotic resistance in SE emergence.
Publications:
A Registry-Based Study Between Human Salmonellosis and Routinely Collected Parameters in Michigan, 1995-2001
A Registry-Based Study Between Human Salmonellosis and Rountinely Collected Parameters in Michigan, 1995-2001
Allele distribution and genetic diversity of VNTR loci in Salmonella
enterica serotype Enteritidis isolates from different sources
Clonal Structure and Variation in Virulence of Salmonella Enteriditis Isolated from Mice, Chickens and Humans
Demographic Risk Factors and Incidence of
Salmonella Enteritidis Infection in Michigan
Epidemiological Attributes of invasive non-typhoidal Salmonella infections in Michigan, 1995-2001
Epidemiology of infant salmonellosis in
Michigan: Records of 1995–2001
In Vitro Attachment and Invasion of HEP-2 Cells by Salmonella Enteritidis Isolated from Human, Egg and Chicken Cecal Sources
Infectious Agents
Multiple-locusvariable-number tandemrepeat analysis of
Salmonella Enteritidis isolates from humanand non-humansources
using a single multiplex PCR
Novel Approaches to Diagnosis Salmonellosis
Risk Factors for Salmonella Oranienburg Outbreak in a Nursing Home in Michigan
The role of neighborhood level socioeconomic characteristics in Salmonella
infections in Michigan (1997-2007): assessment using geographic information
system
